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KMID : 1098420170250040231
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Korean Journal of Medicinal Crop Science
2017 Volume.25 No. 4 p.231 ~ p.237
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Protective Effect of Dopaol β-D-glucoside Isolated from East Asian Monk’shood on Cisplatin-Induced Nephrotoxicity
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Nho Jong-Hyun
Jung Ja-Kyun
Jung Ho-Kyung
Jang Ji-Hun
Jung Da-Eun
Lee Ki-Ho
Kim A-Hyeon
Seong Tae-Kyoung
Park Ho
Cho Hyun-Woo
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Abstract
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Background: Cisplatin is one of the most extensively used chemotherapeutic agents for the treatment of cancer, including bladder, and ovarian cancers. However, it has been shown to induce nephrotoxicity, despite being an outstanding anti-cancer drug. In this study, we investigated the protective effect of dopaol β-D-glucoside (dopaol) on cisplatin-induced nephrotoxicity.
Methods and Results: To confirm the protective effect of dopaol on cisplatin-induced nephrotoxicity, HK-2 cells were treated with 20 μM cisplatin and 80 μM dopaol. Cisplatin increased apoptosis, caspase-3 activity and mitochondrial dysfunction; however pretreatment with 80 μM dopaol successfully attenuated apoptosis, caspase-3 activity and mitochondrial dysfunction. To evaluate the protective effect dopaol on cisplatin-induced nephrotoxicity in vivo, we used an animal model (balb/c mice, 20 ㎎/㎏, i.p. once/day for 3 day). The results were similar to those obtained using HK-2 cells; renal tubular damage and neutrophilia induced by cisplatin reduced following dopaol injection (10 ㎎/㎏, i.p. once/day for 3 day).
Conclusions: These results indicate that dopaol treatment reduced cisplatin-induced nephrotoxicity in vitro and in vivo, and can be used to treat cisplatin-induced nephrotoxicity. However, further studies are required to determine the toxicity high dose dopaol and the signal pathways involved in its mechanism of action in animal models.
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KEYWORD
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Aconitum japonicum subsp. napiforme (H. Lev. & Vaniot) Kadota, Cisplatin, Dopaol β-D-Glucoside, HK-2, Nephrotoxicity
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